Name: dna arrays: methods and protocols (en Inglés)
Price: 133587.00 ARS
Availability: InStock
Author: Rampal, Jang B.
ISBN: 9781617372049

portada dna arrays: methods and protocols (en Inglés)

Formato

Libro Físico

Ilustrado por

Jang B. Rampal

Editorial

Humana

Autor

Rampal, Jang B.

Año

2010

Idioma

Inglés

N° páginas

268

Encuadernación

Tapa Blanda

Dimensiones

22.9 x 15.2 x 1.5 cm

Peso

0.38 kg.

ISBN

1617372048

ISBN13

9781617372049

Categorías

Genética (no médica)
Bioquímica
Biología molecular

dna arrays: methods and protocols (en Inglés)

Jang B. Rampal (Ilustrado por) · Humana · Tapa Blanda

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Reseña del libro "dna arrays: methods and protocols (en Inglés)"

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays--from their conception to their ready off-the-shelf availability--for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .