Part I: Saliva and Other Oral Fluids 1. RNA Sequencing Analysis of Saliva exRNA Karolina Elżbieta Kaczor-Urbanowicz and David T.W. Wong 2. Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry Nagihan Bostanci and Kai Bao 3. Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics Pingping Han, Xiang Li, Wei Wei, and Saso Ivanovski 4. Antioxidant Micronutrients and Oxidative Stress Biomarkers Irundika H.K. Dias, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Iain L.C. Chapple, and Melissa M. Grant Part II: Molecular Biosciences 5. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings José F. Siqueira Jr and Isabela N. Rôças 6. The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes Nicholas C.K. Heng and Jo-Ann L. Stanton 7. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE) José F. Siqueira Jr, Mitsuo Sakamoto, and Alexandre S. Rosado 8. Bioinformatic Approaches for Describing the Oral Microbiota Kristi Biswas, Michael W. Taylor, and David T.J. Broderick 9. Adhesion of Yeast and Bacteria to Oral Surfaces Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, and Ann R. Holmes 10. Quantitative Analysis of Periodontal Pathogens Using Real Time Polymerase Chain Reaction (PCR) Ma José Marin, Elena Figuero, David Herrera, and Mariano Sanz 11. Methods to Study Antagonistic Activities Among Oral Bacteria Fengxia Qi and Jens Kreth 12. Generation of Multi-Species Oral Bacteria Biofilm Models Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, and Gordon Ramage 13. Markerless Genome Editing in Competent Streptococci Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, and Donald A. Morrison 14. A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System Sven E. Niklander and Keith D. Hunter 15. Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients Sai Shyam Vasantharajan, Edward Barnett, Elin S. Gray, Euan J. Rodger, Michael R. Eccles, Sharon Pattison, Fran Munro, and Aniruddha Chatterjee