This second part in the two-volume work Microarrays details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. The two-volume Microarrays presents information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. It provides ample information to all levels of scientists from novice to those intimately familiar with array technology. The first volume deals with methods and protocols for the preparation of microarrays.