DNA origami technology is a growing scientific field that has found applications in chemistry, materials science, physics, biophysics, biology and medicine. This book brings together leading contributors from all fields to explain current developments and to share ideas about future directions. First discussion in the book is about structural design and assembly systems leading to the applications. Interdisciplinary studies are then classified as follows and discussed in detail: DNA origami used for creating new functional materials (combining chemistry and materials science), DNA origami for single-molecule analysis and measurements (as applied in physics and biophysics), and DNA origami for biological detection, diagnosis and therapeutics (medical and biological applications).