Staphylococci constitute one of the most common causes of both superficial and systemic infections in humans. Development of multiple antibiotic resistance among Staphylococcus aureus strains particularly methicillin resistant S. aureus (MRSA) based on the production of alternate penicillin binding protein (PBP2a/2') encoded by mec A gene compounds chemotherapy resulting in treatment failure, increased morbidity and mortality. The study aimed to detect PBP2a/2' among Staphylococcal isolates from clinical samples.